Evidence for production of Indole-3-acetic acid from a fresh water cyanobacteria (Oscillatoria annae) on the growth of H. annus

Cyanobacteria are inexpensive to maintain with high growth rates and produce various biologically active substances like proteins, vitamins, carbohydrates, amino acids, polysaccharides and plant growth regulators. Thus they have the unique potential to contribute to productivity in a variety of agricultural and ecological situations. Indole acetic acid (IAA) is a natural auxin which is also synthesized in many species of non seeded plants, many bacteria, fungi and algae. The amino acid tryptophan is commonly regarded as the precursor for the biosynthesis of auxin in plants. By one pathway tryptophan is converted to indole pyruvic acid via a transaminase reaction, which requires a keto acid and pyridoxal phosphate in addition to the enzyme. Indole pyruvic acid is next decarboxylated to indole acetaldehyde in a reaction requiring a decarboxylase thiamine pyrophosphate. Either an oxidase or a dehydrogenase then oxidizes indole acetaldehyde to IAA. In this study, for the first time an attempt was made to confirm the presence of IAA in the extract of Oscillatoria annae instrumental methods of analysis and a field experiment was also conducted to analyze the efficacy of the extract.